Print ISSN: 1609-5693

Online ISSN: 2410-7409

Volume 31, Issue 2

Volume 31, Issue 2, Summer and Autumn 2007, Page 1-158

Investigation Of Rota Virus In Dogs

Feras N.Hamdaan; Ibtesam K. Hassan; Saleem A.Hasso

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 1-14

397 fecal samples were collected from dogs of different breeds, ages and sex. Detection of rotavirus was achieved by two techniques latex agglutination test and agar gel diffusion test. Blood samples were collected to study various hematological parameters.
The results showed that 95 samples were positive to rotavirus 23 samples (24.2%) were from diarrheic cases and 72 samples (75.8%) were from non diarrheic cases.
Younger dogs were more affected with rotavirus infection 17 positive cases than older ages 6 positive cases. The results showed that diarrheic cases caused by rotavirus were 23 cases (25.3%) that were less than the diarrheic cases caused by other agents 68 cases (74.7%).
It had been shown that the ages less than one year (11 cases) were more affected with rotavirus and complication with dehydration than ages older than one year (2 cases).
It was found that the morbidity rate in males (53%) was significantly higher than females (47%).
The Terrier breed showed a high morbidity rate 42% than others Mixed, Greatdane, Goledretriver and Doberman.
Our study showed that latex agglutination test was more sensitive than agar gel diffusion test and the positive samples out of 200 samples were detected by using latex agglutination test were 39 samples while 18 positive samples by agar gel diffusion test.
There were no differences in blood parameters of the dogs that showed positive results of rotavirus in comparing them with the negative results.
The study showed that rotavirus is widely spreaded among doges with gastrointestinal diseases, it showed that latex agglutination test is more reliable for quick clinical diagnosis.

Biochemical and Haematological Study in Rats Exposed to Cadmium Chloride in Drinking Water

HanaaA.A; Bara N.AL-Ekelly

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 15-29

The objective of this study was investigate the Physiological effect of different concentrations of cadmium chloride in drinking water on some biochemical and haematological parameters of male rats. Animals in this experiment were randomly divided into four equal groups and treated for 15 weeks as follows : Rats in control group were offered ordinary tap water , while animal in M1 , M2 , and M3 were received 10 , 20 , 30 ppm Cdcl2 in drinking water, respectively. The activity of Alanine aminotransferase (ALP), Aspartate aminotrasferase (AST) and alkaline phosphatase (ALP) in serum were measured. Further mor, hemoglobin concentration (Hb) and white blood cells count (WBCs) were detected. The result revealed that addition of cadmium chloride in different concentrations in drinking water caused a significant increase in activity of serum of ALT in treated group (M1 , M2 , M3 ) as compound with control. Within the time the activity of serum AST was significantly increased in three treated group as compared with pretreatment period. On the other hand , significant increase in serum ALP concentration were show in both M1 and M3 treated groups at 12th and 15th week of the treatment as compared with control group. Significant decrease in haemoglobin concentration were observed in M1 , M2 , M3 from 9th week to the end of experiment comparing to control. While cadmium chloride treatment caused significant increase in total white blood cells count in treated groups as compared with control group. On Conclusion, it seems likely that exposure of male rats to cadmium chloride at level above the permissive one had induced clear biochemical and haematological changes.

The Effect of Heat& Cold on The L.monocytogenes in the Milk

Ghazi Mousa Al-Khatib; Mohammed J. Alwan; Nagham M. Al-Jebouri

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 30-38

The influence of heat and cold on the L.monocytogenes in the milk
In order to know the thermal resistance of L.monocytogenesin the contaminated milk, 40gram of powder milk was dissolved in 400ml of heated water.The milk solution was sealed in 40 test tubes 10 ml in each one.these test tubes were divided into 6 groups ,10test tubes in each the 1st,2nd group &5 test tubes in each the 3rd ,4th ,5th ,6th groups,then all the test tubes were inoculated with L.monocytogenes &incubation for 24hrs at 37c.the samples of the 1st &2nd group were dipped in awater bath at tempreture 63c for 30minute &72c for 1-5.5 minute respectively .also the samples of 3rd &4th groups were dipped in awater bath at 72c for 6minute. Test tubes of 1st ,3rd ,5th groups were kept in 4c for variable times while samples of 4th ,6th group were exposured to - 18c for 12days. The results showed no bacterial isolates immediately after heat treatment in all the milk samples of 1st .3rd &4th groups but some isolates were seen after enrichment at 4c&- 18c L.monocytogenes was isolate from all samples of 2nd ,5th &6th groups. The results suugest that cold enrichment play role in repair the thermally injured cells of L.monocytogenes&these organisms surviveve thermal treatment.

Study The Effect Of Feed Withdrawal And Adding Dried Bakery Yeast In The Diet On ph And Some Microbial Types Of Broiler Chickens Intestine

Maha A.AL-Hamiari

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 39-50

This study has been conducted to study the effect of feed withdrawal and adding dried bakery yeast on pH and some microbial indicators which includes Total Bacterial Count (TBC) ,Coliform Count (CC) , Fungi Count (FC) and Lactobacilli Count (LC) in the gastro -intestinal tract of 45 marketed broiler chickens at 8 weeks of age .The birds randomly distributed into three treatments and each treatment to three replicates (15 birds per replicate ) which were: un-treated control (T1) , broiler chickens subjected to feed withdrawal 8 hrs. before slautered (T2) , broiler chickens fed a diet contained 1% of dried bakery yeast three days before subjected to feed withdrawal 8 hrs. before slautered (T3) . The data obtained revealed the following :-
No statically differences in the pH of gastro -intestinal tract were appeared due to feed withdrawal and adding dried bakery yeast . Feed withdrawal (T2) caused a significant (p<0.05) increase in TBC ,TC and FC in the intestine content compaired with T1 and T3 . Adding 1% of dried bakery yeast three days before subjected to feed withdrawal 8 hrs. before slautered (T3) caused a significant (p<0.05) decreased in TBC,CC and FC , in the mean time LC significantly (p<0.05) increased .

Study The Pathological Changes Resulting From Copper Deficiency In Awassi Sheep Organs

Jenan A.Al-Khalide; Saadi AG AL-Sammarraie; Lela M.zake

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 51-60

To induce copper deficiency in sheep (15)Awasi ewes from the college of Veterinary Medicine flock were used. The ewes during the preparation period (18 weeks) were fed on balanced concentrated diet contained (5.2) mg/day copper in addition of the green food.
The ewes were randomly divided into three equal groups for the experimental period (18 weeks). Another balanced concentrated diet with low level of copper (2.6) mg/day was used to feed the primary deficiency group while the secondary copper deficiency group fed on the same concentrate used in the preparation period. The protein ratio and energy level were considered.
The secondary deficiency group received an oral mixture of ammonium molybdate (100) mg with (1) g of sodium sulphate in (100) ml of water daily.
One ewe and two lambs died from the primary deficiency group however a dead still born lamb was born from the secondary deficiency group. The postmortem examination of dead animals studies was done and histopathological samples from the central nervous system, heart, lung, kidney and muscles were examined.
The histopathological examination showed vaculation, degeneration with demylination in central nervous system. These changes were more sever in lumber and thoracic than cervical spinal cord. The same changes were found in the cerebrum and cerebellum. While the liver, heart, lung, kidney showed hydropic degeneration and cloudy swelling. The symptoms and changes through the experimental period were more obvious in the secondary deficiency group than in the primary one.

Using Punica l. Shell Extract As A bacteriostatic For Staphylococcus aureus And Inhancing Locally Processed

Shahrazad M. Al-Shadeedi; Meiad G. Baqir; Faris A. AL-Obaidi; Ali H. Ali

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 61-69

This study was carried out to evaluate Punica L. shell extract as a bacteriostatic for Staphylococcus aureus Invitro , and select the minimal dosage to used as a food additive with species for processing locally sausage and evaluate the microbial and sensory quality .The results obtained revealed that 0.22 % of Punica L. extract was the minimum dose has the effective bacteriostatic for Staph. aureus Invitro and adding this percentage in mixture of locally processed sausage significantly decreased total bacterial count and no growth of Staph. aureus were noticed . No significant differences in the sensory quality of the processed sausage was observed .

The Expermintal Infection of L.monocytogenes &Isolation it from Egg, Oviduct & Ovary

Nagham Mohammed Al-Jeboury

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 70-81

The aim of the present study to detect the transmission of L.monocytogenes from experimental infected chickens to Egg products ,Ovary,Oviduct .&histopathological changes in these organs.
2groups of chickn ,1st group(12 animals)infected with L.monocytogenes 1ml/chick.SC,(1×1010 cfu/ml),2nd group injected 1ml/chick normal salin as control.eggs were collected for isolate Listeria ,animal killed in (7,15,24,30,34,39,42)days,for isolateListeria from ovary,oviduct&samples taken for histopathological examination.
The results show theres no characterstic signs in infected chickens except depression,anoxia&and theres marked decrease of egg production, qualititativly & quantitativly.
L.monocytogenes isolated from egg ( shell, ,yolk,albumine)&persiste to (31,28&19) dayses respectively,also Listeria isolate from ovary&oviduct in chickens which killed in (7,15,24,30)day,¬ isolate from chickens that killed in day (39,34,42)or in control.
The histopathological changes theres congestion of the organs ,diffuse infiltration of infilammatory cells such as lymphocyte , neutrophils & macrophage. also theres odeama, multi necrosis foci &desqumation.

Distribution of helminth infections in gastro-intestinal tract of horses

Azhar Ali Faraj; Fawzia S.Khadim; Shehella R. Fadl

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 82-92

This study concerns with incidence of infection of gastro-intestinal helminthes ( Nematode) in horses. Results revealed that the rate of parasitic helminthes was 50.54%. The peak of Nematode infection was 86.6% in Spring followed by 57.5% .during Autumn then the infection declined in Summer and Winter to 33.73% and 22.5% respectively Four genera of parasitic nematodes were identified the highest rate of infection with Strongylus was recorded in horses 49.54% followed by Parascris 14.09%, the lowest infection with Trichostrongylus and Oxyuris was 9.09 % and 7.72% respectively. Result revealed higher helminthes infections in females 57.05% than in males 39.7%.

Using Modified Anthraxin And Its Evaluation For Testing Cell Mediated Immunity And Studying Histopathological Changes In Lab Animals

am J.L. Al-Jeboury

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 93-103

This study aims to apply a new procedure in preparation of anthraxin from cell wall extracts of a virulent Bacillus anthracis strain 34F2 (Sterne) .Anthraxin was evaluated for potential use as skin testing agent to measure cell-mediated immunity. Guinea pigs were immunized and boosted subcutaneously with Sterne live veterinary anthrax vaccine. Two weeks after the booster dose, animals were skin tested with the antigen which recorded 15.6mm as a mean of erythema and 2.5 mm difference of thickness as compared to the control. Histological changes corresponded with the delayed type of hypersensitivity (DTH) in which the changes involved infiltration of inflammatory cells particularly macrophages, neutrophiles and eosinophils in the skin dermis and in perivascular region in addition to accumulation of fluid (edema) in the dermis and congestion of blood vessels.

Effect Of Listeria monocytogenes Experimentally Infected Dosage In The Percentages Of Broiler Chicks Serum Proteins

Faris A. AL-Obaidi; Muthana M; Nagham M. Al-Jebouri; Shahrazad A. Al-Shadeedi; Eman A. Qazaz

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 104-114

This study has been conducted to study the effect of experimentally infected broiler chicks orally by two dosages of Listeria monocytogenes in the percentages of serum protein of 30 broiler chicks at 7 and 15 days of infection . The birds randomly distributed into three treatments , T1 : control group , T2 : chicks orally infected by 6 X 105 CFU of L. monocytogenes , T3 : chicks orally infected by 34 X 1010 CFU of L. monocytogenes. The data obtained revealed the following :-
Percentages of serum Pre-albumin , Albumin , Total albumins and  - Globulin were decreased significantly (P<0.05) , in the same time Post_albumin ,  ,  - Globuluins and Total globulins increased significantly (P<0.05) in broiler chicks orally by the two dosages of L. monocytogenes (T2 and T3) compaired with control group (T1) after 7 days of infection , whenas a significant (P<0.05) increase in immunoglobulin percentage (- Globulin) with Transferrin in the same time other proteins return to its normal percentages after 15 days of infection , which indicated of development immunity and inhanced chicks health at this age . No differences appeared in chicks serum protein due to the listeria infection dosages in this study .

The Effect of Copper Deficiency on Essential Component Of Sheep Milk

Khulood K.AL-Agealy; Jinan A.AL-Khalidi; Layla M.AL-Kinani

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 115-121

The main objective of this study was to determine the effect of copper deficiency on some milk constituents of ewes.(Thirty)raw milk samples have been collected in the morning and before feeding of the ewes,(10) of them from ewes suffering from copper deficiency and the other(20) milk samples were collected from healthy ewes .The percentages of fat ,protein ,lactose, chloride, specific gravity, moisture and the pH of milk were determined.
The results have shown that copper deficiency have an effect on the milk constituents where all milk samples collected from ewes suffering from copper deficiency had significantly (p<0.01) lower percentages of fat (5.70%) ,protein(3.0%), lactose (3.4%)
and specific gravity (1.0338) than those collected from healthy ewes that had (7.62%),(4.0%),(4.07%) and (1.0369) respectively.
Data revealed that there was a significant (p<0.01) increase in the percentage of moisture (87%) and non significant (p>0.05) increase in the percentage of chloride (0.0921%) in the milk samples collected from ewes suffering from copper deficiency than healthy ones that had (83%) and (0.0858%) respectively. In addition to that no changes in the pH values between all milk samples were noticed.

Estimation of fetal age in sheep by measurement of transthoracic , transabdominal, cotyledon length and width by using real- time ultrasonography

H.M AL-Rawi; S.N. Omran; K. A. Hussein

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 122-132

This experiment includes 118 local Awassi ewes between 2 to 5 years old. The aim of this experiment was to estimate the fetal age by measurement of transthoracic, transabdominal, cotyledon length and width during different period of gestation by using 5MHz real-time ultrasonography. Linear, quadratic and cubic equations were described, which adjust the relationship between the gestation age and each of transthoracic, transabdominal, cotyledon length and width. Results indicated that the quadratic equations of transthoracic and transabdominal were the best equations; while the cubic equations of each of the cotyledon length and width were the best equations to estimate the fetal age.

Isolation &Identification of Newcastle Disease Virus From Wild Pigeons

Anton Sabri AL-bana; Aida Bara Allawe; Ameen Ahmed Sebbar

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 133-140

Isolation &identification of Newcastle disease virus from wild pigeons inhabiting University of Baghdad (Al-Khwarizmi College of engineering) where velogenic and devastating infection of birds was encountered. Chicken embryos fibroblasts (CEF) were used for virus isolation (from infected spleen and lungs) and propagation. Viral cytopathogenic effect was noticed on infected (CEF) cell culture within (24) hr post inoculation. The virus was identified by heamagglutination inhibition (HI) test using reference anti NDV serum.

Genetic evaluation of breeding efficiency estimated by Tomar equation in Holstein cattle

F.R. Al-Samarai

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 141-150

Data analyzed included 13838 records belonged to 4100 Holstein cows over period 1988 to 2005, at the Nasr Dairy Cattle Station.
The aim of the study is to evaluate sires genetically according to their daughters breeding efficiency which was estimated by Tomar equation and to estimate heritability and phenotypic trend for breeding efficiency.
General Linear Model within SAS program was used to investigate the effect of some fixed factors on the breeding efficiency.
The overall breeding efficiency ratio was 79.91% .Component of variance for random effects was estimated by MIVQUE method.

Histopathological study for the effect of vitamin C on the some mice tissues treated with nitrate

R.N.Al-saadi; E.H.Al-taae

The Iraqi Journal of Veterinary Medicine (IJVM), 2007, Volume 31, Issue 2, Pages 151-158

Thirty two mice were used to study the histopathological changes resulted from the effect of vitamin C on the some mice tissues treated with nitrate.
Mice were divided randomly in to four equal groups. The 1st group was given Vit C and potassium nitrate via water. The 2nd group was given potassium nitrate and the 3rd group received Vit C only, while the 4th group represented the control.
Two animals from each group were scarified weekly. The results showed similar pathological changes in the 1st and 2nd group especially in the stomach and intestine which gave severe changes that characterized by hyperplastic changes in addition for the hemorrhages and the infiltrations of inflammatory cells in all layers of both organs. Less alteration was seen in the liver and kidney. On the other hand, no pathological changes in the 3rd and 4th group were recorded.