Print ISSN: 1609-5693

Online ISSN: 2410-7409

Volume 36, Issue 2

Volume 36, Issue 2, Summer and Autumn 2012, Page 50-253

Effect of Some Parameters in Pellets Production Used for Fish Feeding

M. Sh. M. Al-Khshali

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 50-53

The study dealt with the usual fish feed used in fish farms by changing its moisture content from 500 to 600 ml / kg and using three different hole diameters (2,4,6 mm) in manufacturing the pellets. These two variables were studied concerns the effect on productivity and expansion ratio of the pellets, their density and its settling velocity in water, by using the factorial experiment under Completely Randomized Design (CRD) with three replicates and significant effect tests between treatments under (LSD) test at (0.05).The Results showed that with increase mash of diet moisture content from 500 to 600 ml found significant effect in productivity and it provided maximum productivity (27.99 kg/h) with moisture content 600 ml and it significant effect in settle velocity and was the least settle velocity (7.65 cm / s) at moisture 600 ml and results study not found significant effect in expansion ratio and density for pellets with the changing the product holes diameter from 2 to 4 to 6 mm found significant effect in productivity it up was (38.50 kg / h) at hole diameter 6 mm and also found significant effect in expansion ratio and density and settle velocity for pellets it was up the density (1.092 gm / cm3) and the least expansion ratio (-3.14%) and the least settle velocity (6.18 cm/s) at the holes diameter 2 mm.

Effect of Pellets Diameter and Mash Moisture Content in Pellets quality and production for fish feeding

Abdul-Khaliq A. F. Ahmed Ali

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 54-59

This research included studying some of factorial conditions manufacture of pellets are included pellet diameter by using three holes diameters for the pellets mincer (2 , 4 and 6 mm) and mash moisture content from determining two levels are (500 and 600 ml) for 1 kg from mash and effect this factorial in specific capacity and specific energy and also pellet settling velocity and water stability. The studying carried employment Completely Randomized Design (CRD) with three replications. The results showed with increase pellet diameter from 2 to 4 to 6 mm apparent. Significant effect in increase specific capacity and settling velocity. Also results to decrease specific capacity 37.87 kg / kwh and minimum specific energy 26.43 kwh/ T at pellet diameter 6 mm, and the minimum settling velocity 6.18 cm / s and maximum water stability for pellet it was pellet diameter 2 mm with increases mash moisture from 500 to 600 ml significant effect in increase specific capacity and water stability and settle velocity and was maximum specific capacity 27.39 kg/kwh and maximum water stability and minimum specific energy 40.39 kwh/ T with 600 ml mash moisture and minimum settling velocity 7.65 cm / S at 600 ml mash moisture content .

The Inhibitory Effect of Cinnamon (Cinnamomum zeylhnicum) and Ginger (Zingiber officinal) in the Growth of Some Bacterial Species in the Gastrointestinal Tract of the Quail

Husam H. Nafia; BaKer T. Jaber; Nuha I. Hasan

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 60-64

This experiment conducted for studying the effects of crushed cinnamon and ginger to the diet at levels 0.1, 0.3, 0.6% in the bacterial growth of gastrointestinal tract of the quail. Ninety birds one day age divided randomly into ten transactions as 9 birds per treatment (three birds per duplicate). The numbers of bacteria, total aerobic bacteria in the colon of feces of birds were calculated, the results showed a significant reduction in the number of aerobic bacteria and the numbers of bacteria in the colon. Proportionally increase in the levels of crushed cinnamon, ginger, individually or in combination in comparison the control group showed influence inhibitory to the numbers of bacteria in the colon and aerobic bacteria when you add different levels of transactions compared to crushed ginger and cinnamon mixture into the bush.

Detection of Sarcocystosis in some wild birds

May H. Kawan; Azhar A. Faraj

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 65-70

The aim of this study is to detect the prevalence of macroscopic sarcocystosis of 480 wild birds ( 82 Pygmy cormorant, 34 Black Stork, 99Common Gull,74 little Egret, 91Raven and 100 Mallard) were hunted from October 2009 to September 2010 in Al Rashdia areae /Baghdad . The macroscopic infection total was 9.58%. it was regarded to monthly variation. It was 27.5% in February and 2.5% in August. The highest infection rate was in the thorax muscles (6.2%) and the lowest in the neck muscles (1.6%), Also the macroscopic infection rate was higher (13.5%) in males than females (5%).

The Relationship between ELISA and HI Tests for Determination the Antibodies of Rubbela

Layla Fouad Ali; Ghanima Sadik M

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 71-74

The study includes (44) blood samples were vaccinated with MMR vaccine. The IgM antibodies of Rubbela Virus were measured by HI test, 22 samples were positive and 22 were negative. These samples were tested by Enzyme Linked Immuno Sorbant Assay ELISA test to determine the relationship between them. The results showed that ELISA test was more sensitivities than Haemagglutination inhibition HI test in determination of rubella specific antibodies, so it is better than HI test in measuring the immune-status of individuals vaccinated with rubella vaccine and there was a positive relationship between ELISA test and HI test and was detected (r= 0.585).

Effect of magnetized water in blood picture of common carp Cyprinus carpio infected with Aeromones hydrophila

Khalid A. Rasheed; Abdulmotalib J. Al-Rudainy; Eman S. Khamees

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 75-82

A total of 80 specimens of common carp Cyprinus carpio were used in present study ranges between 142-156 g with 150 g in average body weight, and between 22-27cm with 25cm in average body length to investigate the effect of magnetized water in blood pictures of fish as well as Aeromonas hydrophila pathogenic bacteria. Fish were randomly distributed using aquarium with dimension 70X40X40 cm upon four treatments, with two replicates for each treatment using magnetized water with different intensity ( 500 , 1000 , 1500 gauss) , while the control treatment free of magnetized water through the period of four weeks . Results shows that the bacteria count were decreased in such magnetized water in comparison with control treatment . The results of statistical analysis show significant differences ( P≤0.01) between all treatments . Results of blood picture test show significant differences ( P≤0.01) between test ,the three treatments containing magnetized water compared with control treatment .It can be conclude that the possibility of using magnetized water in prevention and control of some bacterial diseases in common carp.

Histological Studies on the Retina of the Falcon "S Eye Ball (Circus Cyaneus C.) Under Light and Electron Microscopy

Jehan Mahmood Rajab; Shakir Mahmood Mirhish; Samawal Jassim Mohamed Al-Robaae

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 83-92

The histological study showed that the retina of falcon"s eyeball was thin at the periphery and ranges between 0.6-0.8 μ but it was thick at center 1.68-2.64 μ. The retina consists from ten layers: pigmented epithelium, rods and cons layer, external nuclear layer, external plexiform layer, internal nuclear layer, internal plexiform layer, Gangilionic cells layer, neurofibres layer and internal limiting membrane layer. The ultra-structural study stated that the rods and cons layers contained single rods with single and double cons. The retina characterized by lacking of the oil droplets in the internal segments of rods with narrow external plexiform layer in order to form network connecting rods and cones, horizontal cells, bipolar cells and Muller's cells.

Epidemiological study of Setaria equina infection in donkeys

Athmar .K. AL-Azawi; Amer Rasool Fadhl; Shahela Rasool Fadhl

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 93-97

Autopsy study of 218 (106 male and 112 female) animals slaughtered in Baghdad ( Al-ZAWRA ) Zoo, of central Iraq (Diyala, Wasit, Baghdad) .The total infection rate of Setaria equina was 11.9% and the highest infection rate (22.2%) was recorded in October, where is no infection in January, February and August. It has been observed that the high infection rate (14.5%) during the months of spring and autumn (18.8%) and decrease in winter and summer 3.6% and 10%. Female donkeys with a higher proportion (13.5%) than male10.3% respectively differences ( P < 0.05 ).Worm intensity ranged between 1-6 worm and was shown high worm burden in Autumn and Spring fallowed by Summer and finally Winter seasons, which no infection was recorded in some months of it

Effect of sweet almond suspension as anti-inflammatory in experimental infected mice with arthritis

Lubna A. Kafi; Zobahi; Yousif Z. A. AL

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 98-105

The current study was conduct to determine the effects of oral treatment of sweet Almond Suspension (SAS) on induced arthritis by Incomplete Freund’s Adjuvant (IFA).
Seventy mice, with close age and weight were used; they were equally divided in to 7 groups (10 mice per group). The first group served as negative control (non infected – non treated (NINTC). The second group was the positive control (infected non treated, (NINTC) the third and fourth groups were those treated with 1.42 or 2.84 g/kg of SAS respectively. The fifth group was treated with voltarin (ITV), while the sixth and seventh groups were treated with the same closes of SAS but before infection (Prophylactic infected groups, PI1, PI2).The size of knee joint, carrageenan test, level of alkaline phosphatase and histopathological changes in the knee joint used as parameters to compare between groups. The results showed that SAS was able to subside signs of arthritis by decreasing the size of knee and decrease the formation of edema which was induced by injection of carrageenan in the paw of the animal, Histopathological study showed that joints of treated groups by SAS had no signs of arthritis. However, there was slight infiltration of netrophile in treatment and prophylactic group.

Effect of Adding Different Concentrations of Sodium Hypochlorite in Drinking Water on Blood Serum Albumins and Globulins of Broilers

Atika A. R. Al-Hemairi

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 106-110

The study was conducted to investigate the effect of different concentrations of sodium hypochlorite (sodium dichloro iso cyanurate) in the drinking water on the percentages of serum proteins of broilers chicks at 4 and 7 weeks of age. Two hundred forty Hubbard chicks one day – old aged were divided randomly into four equal groups, each group was divided into two replicate. The four groups were as follows: Treatment 1: Adding 6.68 mg /liter water of sodium dichloro iso cyanurate to drinking water. Treatment 2: Adding 13.36 mg /liter water of sodium dichloro iso cyanurate to drinking water. Treatment 3: Adding 20.04 mg /liter water of sodium dichloro iso cyanurate to drinking water. Treatment 4: Supplying water without any additive (control 1). The results revealed that: Adding sodium dichloro iso cyanurate (T1, T2 and T3) increased significantly (P<0.05) broilers blood serum albumin , total albumins , - globulin and total globulins at 4 and 7 weeks of age, in the same time no differences appeared in the others compared with control group . this indicated of the importance of adding sodium dichloro iso cyanurate to enhance broilers health.

Effect of Bay leaves (Laurus nobilis) Plant Extracts as Anti-Oxidant on Crud Sun flower Oil

Saba J. Ajeena

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 111-118

This investigation was carried out to study the Bay leaves (Laurus nobilis) and extracted three types of Aqueous, Ethanolic alcohol, Essential oil extracts of leaf plant powder study. The performance of extracts as anti-oxidant agent were evaluated by estimate the value of Thiobarbituric acid (TBA) of sunflower oil to these extracts in concentrate% 0.04 and %0.02 compared with commercial antioxidant BHA (Butylated hydroxyl anisole) and BHT (Butylated hydroxyl toluene) in concentrate % 0.02 , in addition to the control sample (of crude sunflower oil without any additives) during 16 days storage at 60 C. The results showed an increase in the TBA values in sunflower oil, crude and stored in during storage at 16 days in 60 C. Some extracts laurels showed high anti-oxidant activity and more than commercial antioxidants (BHT) and (BHA). The results of the TBA transactions for crude extracts of plant concentration% 0.04 most efficient overall transactions in concentration % 0.02, aqueous extract of bay leaves in the concentration of % 0.04 showed high activity as an antioxidant with 2.88 mg Malonaldehyde / kg oil TBA value in the 16th and the last day of storage time in 60 C, while the TBA values of the rest of the coefficients of crude sunflower oil translate with plant extract :essential oil ethanolic alcohol and water extract in the concentration of 0.02% were 4.04, 3.39, 3.12 mg Malonaldehyde / kg oil, and in the concentration of 0.04% for essential oil ethanolic alcohol treatment were 4.01 and 2.29 mg Malonaldehyde / kg oil , respectively in the last day of storage, either control and commercial antioxidants BHA and BHT reached TBA values of crude oil 6.88, 3.16 and 3.50 mg Malonaldehyde/kg oil, respectively, in the same day of storage. Was estimated as the content of plant nutrients and mineral major and minor, were detected. Chemical quality of the active chemical compounds of plant leaves, which included tannin, Alclaicosadat, Alsabonyat, resins, phenols, flavonoids and alkaloids were evaluate to.

Study the Anatomical Descriptions and Histological Observations of the Kidney in Golden Eagles (Aquila Chrysaetos)

Ramzi Abdul ghafoor Abood AL-Agele

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 145-152

The present study is preliminary investigate of the kidney gross morphology and some histological observations was studied in golden eagle (Aquila Chrysaetus). The anatomical descriptions of the kidneys had revealed a paired kidney and ureters were protrude, flattened and extra-peritoneal organs. The kidney tissue is transverse by major nerve trunks and blood vessels, securing the kidneys tightly in place. Each kidney consists of three lobes: cranial, middle and caudal lobes. The cranial lobe was the largest and wider than the other two lobes and the caudal lobe was appeared similar to middle lobe but little smaller than it. There is no line of demarcation between cortex and medulla as in mammals. The glomerulus consisted of a tightly packed central core of mesangial cells, surrounded by capillary loops. The cytoplasm of the proximal convoluted tubules cells contains condensing vesicles and a large apically situated nucleus. The distal convoluted tubules and collecting ducts were distinguished on a topographical basis revealed that varied slightly in their reactions with PAS stain and appeared as vacuoles or look like vesicles contains secretion from lining epithelial cells of distal convoluted and collecting tubules which appeared diffused in cross section. The cells of distal convoluted tubules and collecting tubules possessed vesicles with a clearly defined coated outer membrane and some had small blebs invaginated membrane. However in this study found vesicles or vacuoles in the lumen of collecting tubules revealed the secreting cells had margins that were often incompletely membrane –bound and continuous with the apical cell margin as if discharging their contents to the tubular lumen.

A retrospective study using IgA in association with IgG and IgM for precise diagnosis of toxoplasmosis in suspected cases

khitam; Y.O. AL-dujaily; Athmar k.Alazawi; Haider; K .Shindakh; Jaber; A.M.Taqi

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 153-157

In order to achieve precise diagnosis of toxoplasmosis in suspected cases of aborted women and infants with abnormal clinical manifestation, 1527 serum samples consisting of 1450aborted women and 77 infants were tested by enzyme linked immune sorbent assay (ELISA), using IgG, IgM and IgA platellat oxogondii kits, 865 (56.64%) were found positive, 848 ( 58.48% ) were found in aborted women and 17 (22.07%) infants with a significant differences (P≤ 0.05).The immunoglobulin IgM , were detected in 72 (8.49%)cases of aborted women, IgM with IgG were found in 44 (61.11%) and with IgA in two (2.77%) cases ,whereas found in three(17.64%)cases of infants. While IgA were found in 77(9.08%) cases of aborted women, and 6 (35.22%) infants. IgG was detected in699 (82.42%(with a significant differences(p≤ 0.05 ),alone in602 (86.12%) ,and with IgA in 34(4.86%) case of aborted women and 8(47.05% )of infants. These results were support the importance of testing the suspected cases by all kinds of immunoglobulins (IgG, IgM and IgA) to achieve precise diagnosis for toxoplasmosis.

Clinical study of experimentally induced vitamin E and selenium deficiency in Awassi ewes and their newborn lambs

A.A. AL-Ani; A. M. H. Judi; H.K. Abood

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 158-162

Experimental induction of vitamin E and selenium deficiency by deficient diet was carried out on Awassi ewes and their newborn lambs. The clinical signs were characterized by sudden death in 4 lambs out of 14 lambs in deficient group and other lambs showed a variable signs included inability to suckle, arched back, weakness, dullness, emaciation and recumbency. Serum selenium and vitamin E levels of these lambs were 0.01 ppm and 0.34 mg/L respectively. The clinical signs in ewes included loss of body weight and loss of wool, Weakness, dullness and recumbency. Serum selenium and vitamin E levels of these ewes were 0.02 ppm and 0.61mg/L respectively. It was concluded that vitamin E and selenium are essential antioxidants and their deficiency exposes the sheep industry to many serious losses.

Assessment the Antioxidant and Hypolipidmic Effect of Black Cumin (Nigella sativa L.) Flavonoids in Induced Oxidative Stressed Male Rabbits

Kahtan A. Al-Mzaien

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 163-173

Qualitative and quantitative assessment of flavonoids, sugar moiety and antioxidant scavenging activity (in vitro and vivo) of black cumin seed ( Nigella sativa L.) as well as serum lipid profile inhydrogen peroxide (H2O2) induced oxidative stress and flavonoids treated adult male rabbits were the main aim of the current study. The results showed that black cumin seeds contained 4.50gmcrude flavonoids per kg crushed seeds and the high performance liquid chromatography indicated the existence of 22 flavonoids, only seven of which were identified flavonoids represented nearly 2.751gm/kg of the total flavonoids. The concentration of kaempferol was the highest whereas quercetin the lowest. Glucose, fructose and sucrose were the sugar moiety of flavonoids with an average concentration of 3.22, 2.21 and 3.31 g/kg crushed seeds respectively. Assessment the antioxidant scavenging activity of the extracted flavonoids and its effect on lipid profile in H2O2 treated rabbits were carried out as follows; eighteen adult male rabbits were randomly divided into three equal groups. Rabbits in the first group were received tap water and served as control (C), whereas animals in 2nd group received 0.5% H2O2 in drinking tap water (T1), animals in the 3rd group were received 0.5% H2O2 in drinking water with 27.5mg/kg B.W. of black cuminseeds flavonoids.Fasting blood samples were collected at 0, 4, 8 and 12 weeks of the experiment. The results clarified that 0.5% H2O2 in drinking water caused significant elevation in serum malondialdehyde (MDA) and reduction in glutathione (GSH) concentration of treated animals respectively.The incidence of antioxidant scavenging activity in vitro was more pronounced than that recorded in vivo particularly at the early stages of administration and extracted flavonoids ameliorate to a great extent the incidence of hydrogen peroxide induced oxidative stress in male rabbits. The results revealed that administration of 0.5% H2O2 in drinking water for 12 weeks to male rabbits caused significant p<0.05 elevation in TC, TAG, LDL-C and VLDL-C concentration, with significant p<0.05 reduction in serum HDL-C concentration as compared with the control where as Black Cumin seed flavonoids caused significant reduction in the serum concentration of TC, TAG, LDL-C, and VLDL-C and significantly p<0.05 elevation in HDL-C concentration comparing to H2O2 treated group.
Conclusion: This study clarified the significant role of black cumin flavonoids in modulation of glutathion (GSH) content and malondialdehyde( MDA) equivalent and exhibited highly hypolipidmic effect.

Effect of Varying Doses of Estradiol Benzoate on the body weight and blood picture in Turkeys

Saad J. Gatie; Bassam A. Al-Shimmary; Mohammed S. Almuslehi

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 174-179

A present study was carried out to investigate the effects of Estradiol benzoate 2 (mg/ml) hormone doses on the body weight and blood picture of Turkeys birds through studying the following parameters: body weight, RBC count , total WBC, MCV, MCH, MCHC, and platelet PLT. Forty growing Turkeys (30 days age) weighting between (250-350)gm were randomly divided into four groups (ten each group) treated as follows: Turkeys in the first group were injected with single dose of Estradiol benzoate (1 mg) on the first day of experiment (30 day post hatching) subcutaneously in the neck and termed as G1 group, the Turkeys in the 2nd group (G2) were injected Estradiol benzoate (2 mg) on the first day of experiment subcutaneously in the neck while the Turkeys in the third group (G3) were injected Estradiol benzoate (0.3 ml) at the first day of experiment subcutaneously in the neck the fourth group (G4) served as control, body weight was determined on (0, 15 and 30) days of experiment and blood samples were collected on 15th and 30th day of the experiment from groups to evaluate the blood parameters. Results revealed a significant increase (P<0.05) in body weight of G1, G2 and G3 in a comparison with control group at (15 and 30) days of experiment. Furthermore, in comparison among treated groups G2 and G3 also showed a significant increase (P<0.05) in the body weight as compared with G1 group during periods. Moreover, data revealed that all treated groups showed non-significant differences in blood parameters experiment as compared with control group.

The effect of some environmental factors that affecting daily milk yield of Iraqi buffaloes in Ninewah

Ali S. Sadiq; Nassar N.Al; Anbari; Odday S. Al; Obaddy; Haddad; Alaa S. Al; Garabed A. Avadesian

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 180-186

This study was carried out on the local buffalo in Ninewah, data were collected from154 buffalo in two herds during the period 1/7/2010 until 18/7/2010 in which milk collected daily in sequence ( 1st day , 5th day , 9th day , 13th day and 17th day ) and recording maximum and minimum temperature .Data were analyzed using general linear model ( GLM) within SAS program to study the fixed effects ( parity , herd , stage of lactation and test day ) , and regression coefficient with heritability . Overall average daily milk yield was 9.69 ± 0.12 kg and it appeared that parity , herd and stage of lactation has a highly significant effects in daily milk yield , while test day recorded no significant effect in the above . Minimum temperatures ranged (23.5 – 27.5 °C) and maximum was (41.8 – 45.6 °C). Regression coefficient for daily milk yield on maximum temperature was -0.259 kg / °C on (P > 0.01) and for minimum was 0.0325 kg / °C and this was non - significant, while the prediction equation (ŷ) was:
Y^ (max) = 21.121 – 0.259 (X1)
Y^ (min) = 8.863 + 0.0325 (X2)
The heritability estimate for daily milk yield was ranged between (0.17 – 0.21) for the test day (recording day) , it was concluded that from this a number of fixed effects , and yield was decreased significantly with rising in ambient temperature and the estimates of heritability for daily milk yield belonged to test day was rather low.

Protective Effect of Alcoholic extract of Black Current in Male Reproductive System of Methionine Overload Rats

Baraa Najim Al-Okaily

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 187-194

This study was undertaken to investigate the deleterious- effect of methionine overload on male reproductive system of adult male rats. It was also aim to studying the beneficial protective effect of alcoholic extract of black current. Eighteen adult male rats (175-200 gm) were used, animals were randomly divided into three groups (6/group) and were treated daily for 42 days as follows: 1-Group C (control); 2- group I(GI), the rats in this group were orally intubated by gavages needle DL-methionine (100 mg / Kg BW.) 3- animals in group II(GII) were administered orally alcoholic extract of black current (60 mg /Kg BW.) in addition to methionine. At the end of the experiment blood samples were collected for measuring serum testosterone (T) hormone and follicular stimulating hormone (FSH) concentrations. As well as histological section, for testis was taken for histopathological study the results revealed that oral intubation of 100mg /kg BW. of methionine for 42 days caused significant decrease - in serum testosterone (T) hormone and follicular stimulating hormone (FSH) concentrations in groups (GI and GII) comparing to control ,while oral intubation of alcoholic- extract of black current (GII) caused significant elevation in the concentration of these two hormones comparing to methionine treated group (GI). Furthermore, methionine intubation caused significant decrease in diameter and thickness of seminiferous tubules with cellular degeneration of Sertoli cells, while black current intubation caused significant increase in the previous criteria and regeneration of Sertoli cells. In conclusion, this study pointed to the deleterious effect of methionine overload and assures protective effect of alcoholic extract of black current on adult male rats reproductive system.

The Effect of Folic Acid Administration of Some Physiological Parameters in Normal Adult Male Rabbits

Sabreen Majeed Mohammed Ali

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 195-198

This study was conducted to find out the effect of folic acid at different doses (5 g and 10g/animal daily) on blood parameters in adult male rabbits. The animals were divided into 3 equal groups each group contained five animals. One of them as control and other as treated groups T1 and T2. The experiment lasted four weeks. GroupT1 got folic acid at dose 5 g/daily/animal and GT2 10g/animal. To study the hematological parameters such as [total red blood cell (RBC), total white blood cell (WBC), packed cell volume PCV, hemoglobin concentration (Hb) and using serum to detect total serum protein. The folic acid was causing significant increasing in most of these parameters due to the stimulating effect of it and anti-oxidant properties that protect the cell membrane from free radical. Therefore, the administration of folic acid is useful especially for children, pregnant women and aging people.

Study the effect of addition alcoholic extract of Tribulus terrestris on bovine oocyte maturation in vitro (IVM)

Imad M. AL-Maeeni; Khulood W.AL- Samaree; Liqaa Y. Abdul

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 199-203

The present study included 98 bovine ovary collected from AL-Shulla slaughter house immediately after the animal was slaughtered. Ovaries were preserved in physiological saline at 37 Co and transport to the laboratory within 3-4 hrs. Diameters of the follicles were measured and divided into large (6-10 mm) and small (1-5 mm) follicles. Cumulus-oocyte complexes (COCs) were aspirated from large and small follicles using a 18 G needle connected to a 10 ml syringe COCs surrounded by compact and thick cumulus cell were cultured in an integrated culture media RPMI-1640 , than were divided in to two groups , the first group (control media) using integrated culture media only , the second group alcoholic extract of Tribulus terrestris (Tt) plant was added to the culture media RPMI-1640 once a concentration of 50µg/ml , and the other a concentration of 25µ/ml . The results of the current study demonstrated superiority of alcoholic extract of 50µg/ml in the percentage of oocytes maturation which optended or drawn from large and small follicles compared with a concentration of 25µg/ml of the alcoholic extract it was observable the effect of adding alcoholic extract of T. t. plant in two concentrations of 25µg/ml and or 50µg/ml on the percentage of mature oocytes compared to control media (culture media RPMI-1640) .The present results indicated that the oocytes retrieved from larger follicles is better than small follicles in maturing oocytes maturation. In conclusion, alcoholic concentration of 50µg/ml preference extracted first and then the concentration of 25µg/ml T. t. plant extract that was added to culture media RPMI-1640 , superiority in percentage of the mature oocytes compared to control culture media RPMI-1640 , also the large follicle were better than small follicles in terms of equality suitable for mature oocytes.

Monthly changes in testes and epididymis measurements with some semen characteristics of tail epididymis for Iraqi buffalo

Mohammed Mehdi AL-Sahaf; Najlaa Sami Ibrahim

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 204-208

The present study was carried out to investigate the reproductive activity of mature buffalo bull and months changes on the testes and epididymis. Two hundred and fifty ( 250) testes of mature buffalo bull were collected from the slaughter house east Kerbalaa city from December/2010 to September2011 ,visual examination of the male before slaughter was done to make sure it is healthy. Semen was collection from the left testes before slaughtering via aspiration from the tail of epididymis and make semen evaluation individual motility, viability, concentration and abnormalities. After slaughtering the right testes measuring the length and width then carefully dissected the epididymis and measuring the length and weight of epididymis and measuring the weight of testes, diameter and weight of epididymis tail. The present results demonstrated increased (P<0.05) significantly in weight, length and width in testes in March and April, and increased significantly in length, weight of epididymis, diameter and weight of epididymis tail in April and May. The individual motility higher significantly P<0.05 in December January and April, and the live sperm higher significantly in April, March and May, and the concentration higher significantly in April , May and March, while the abnormalities was higher significant in August ,July and September. From all these results conclusion reproductive activity of buffalo bull and semen physical characteristics increased in the moderate and cold months and decreased in hot months .The increased of ambient temperature in hot months lead to disturbance in reproductive activity but don’t stopped it .

A study of some physiological aspects in mature male rabbits after oral administration of Citrullus colocynthis

Saad S. Al-Dujaily; Fetiwa Manwar; Salam H.Ibrahim

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 206-216

The goal of this study is to determine the effect of Citrullus colocynthis (CC) fruit (bitter apple) on some physiological aspects; the body weight, hematological parameters and the function of thyroid gland of male rabbits as a model for mammals. Two experiments were accomplished on sixty mature male rabbits that equally (30 animals in each experiment) and randomly divided into six groups each one contains five animals. In the experiment No.1 and experiment No.2 , independently, three groups were considered as treated groups in each experiment ( represented as T1, T2 and T3 groups ) and daily orally administrated low dose of CC (4.8mg/Kg/day, experiment No.1) for three groups in experiment No.1 and double dose of CC extract (9.6mg /Kg /day , experiment No.2) for 2, 4 and 8 weeks for the three groups in experimentNo.2,respectively. The other three groups in each experiment were considered as control (C1, C2 and C3 groups, respectively) groups were given orally distilled water (DW) at the same periods of treated groups. Different hematological parameters and thyroid hormones were studied. There was a significant (P<0.05) increase in the body weight gaining, with no significant changes in RBCs, WBCs, and Hb concentration through the first two weeks in all the treated rabbits compared to before treatment and control groups. Results of this study revealed that all the groups showed a significant (P<0.05) increased in the serum triiodothyronine hormone level and a decrease in the serum thyroxin hormone level of the thyroid gland when both doses were orally administrated through different periods of treatment compared to control groups. It is concluded that low dose of CC treatment has an advantageous physiological effect on the body weight and triiodothyronine hormone with no changes in certain hematological parameters through first two weeks .The significant differences that found following 4 and 8 weeks of CC treatment were within physiological normal level, and accordingly with the dose and treatment periods in rabbits.

Effect of Aqueous Extract of Tribulus terrestris orally administration on some Biochemical Parameters in Castrated Male Rabbits

Abeer Uthman Moosa; Rasha Hussein Kuba; Yasser A.H. AL- Issa

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 217-221

The present study is aimed to investigate the influence of an aqueous extract of Tribulus terrestris on some biochemical parameters namely, alkaline phosphatase (ALP), Iron, phosphate (PO4), sodium (Na+), potassium (K+), aldosterone, and cortico steroid in unilateral and bilateral castrated male rabbits. Thirty mature male rabbits which are divided randomly in to six groups. group 1: is control, group2: orally administrated 150 mg / kg / day of an aqueous extract of T. terrestris, group3: is bilateral castrated rabbits received D. W., group4: is bilateral castrated rabbits received (150 mg / kg / day) of the extract, group 5: unilateral castrated rabbits received D. W., group 6 is unilateral castrated rabbits received 150 mg / kg / day of the extract, all doses were given orally every day for 30 days. At the end of the experiment, sera were collected and biochemical tests measured. The results showed a significant (P<0.05) increased in ALP level in group 2 and in PO4 level in group 5 in comparison with group1, while PO4 level tended to be decreased significantly (P<0.05) in group 6 when it compared with group 5. The results also showed a significant(P<0.05) increased in (K+) and (Na+) in group 2 and group 6 in comparison with group1 while there was a significant(P<0.05) decreased in (K+) and (Na+) in group 4 when it compared with group 2. In addition there was a significant (P<0.05) increased in aldosterone concentration in group 6 compared with group 1 and group 2. While there was a significant decreased in cortico steroid concentration in group 3 , 5 and 2 compared with group 1 but the cortico steroid showed a significant (P<0.05) increased in group 6 compared with group 1. There were no significant differences between groups concerning the iron concentration.

Detection and study of the experimental infection of Aeromonas strain in the common carp (Cyprinus carpio L.)

Sundus A.A. Alsaphar; Jamal K.H. Al-Faragi

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 222-230

The aim of this study was to isolate motile aeromonad strain of common carp (Cyprinus carpio) which was obtained from farms around Baghdad and identification of the isolate by using Analytical profile index API20E. Experimental infection was carried out by intramusculer injecting LD50 does 0.3× 108.66 cell/fish into common carp Cyprinus carpio L. Infected fish after 14 days of injection showed hemorrhage and ulceration on the body surface, eye abnormalities and accumulation of red-colored ascetic fluid . Macroscopically pale liver with hemorrhagic and necrotic spots was seen and kidney showed liquefaction. Microscopically severe necrotic changes in muscular structure and internal organs with intense polymorphonuclear cell. Infiltration characterized by of pyknosis of nuclei with presence of cellular detritus were seen.

Assessment the therapeutic effects of aqueous extracts of Cilantro and Garlic in mercuric chloride poisoning in rats

Eman H.Y.Al Taae; Rajiha A. AL. Naimi; Ghusoon A.K. AL-Neamah

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 231-243

The objective of the present study was to evaluate the therapeutic effects of cilantro and garlic aqueous extracts in toxicopathological changes due to acute and chronic mercuric chloride poisoning in rat. The acute toxic signs appeared at short time after treatment with mercuric chloride. Severity of intoxication and mortality rate were proportional to the dose of mercuric chloride given. Chronic clinical signs were checked continuously which characterized by anorexia, body weight loss, pale –yellow mucous membranes, rough skin, ruffled hair, dyspnea and hemoglobin urea the severity of signs was dose dependant. Results showed severe toxicopathological changes in kidneys, liver and brain with precancerous lesions in liver and fore stomach, with residual accumulation of the compound in these organs reaching the highest rate in kidneys in both acute and chronic toxicity. Treatment with plant extracts resulted in decreasing the severity of the pathological changes in the mentioned organs and increase in the immune response of the body especially in the animals treated with combination of cilantro and garlic extracts together. This was reflected in decreased levels of compound accumulation in tissue. It could be concluded that mercuric chloride poisoning in rats causes toxicopathological changes in the kidneys, liver and brain with precancerous lesions in liver and highest residual accumulation in kidneys .Treatment with cilantro and garlic extracts could reduce the severity of these lesions and residual accumulation with elevation in the immune response.

Effect of Environmental High Temperature on the Reproductive activity of Awassi Ram Lambs

Hassan Falah Kashef Al-Ghetaa

The Iraqi Journal of Veterinary Medicine (IJVM), 2012, Volume 36, Issue 2, Pages 244-253

The aim of this study was to detect the influence of high temperature during summer and early autumn seasons on the reproductive activity of Awassi ram lambs. Study conducted in Baghdad from June 1st 2011 to Nov. 30th 2011, using thirty Awassi ram lambs aged 11.5±1.5 months. Testis, Seminal vesicle gland and bulbourethral gland dimensions and weights were taken once monthly, cortisol and testosterone hormones were estimated, percentages of dead and abnormal sperms were calculated and histopathological examination was applied on the testes. The results revealed a significant (P<0.05) effects of hot season on the genital organ dimensions and weight, and on testicular histological texture leading to sloughing and degenerative changes in seminiferous tubules which causes elevating significantly (P<0.05) in percents of abnormal and dead sperms during mid-summer in comparison with fall season. There was decline in testosterone hormone level, while the cortisol hormone levels remains without significant differences. In conclusion: Awassi ram has a great ability to heat stress adaptation to decline the effect of external hyperthermia on reproductive ability as soon as possible, and can resume almost a perfect sexual activity with high fertility rate immediately at the regression of climatic high temperature.